fv10i manual

Cancer Research Cell Culture Developmental Biology Drug Discovery Drug Discovery Fluorescence Live Cell Imaging Molecular Cell Biology Neuroscience Regenerative Medicine Clinical Research. Microscope Classroom Ask the Experts Learn about microscope (Japanese text only) Terminology Scientific Articles Video Articles Application Notes Microscopy Resource Center The Olympus Museum: Microscopes Support.The completely re-engineered design of this microscope, integrated into a self-contained package with a variety of functions, enables even inexperienced and first-time users to perform easily and efficient high-quality confocal imaging. Olympus has made no compromises in ergonomics and image quality, using high-quality optical components and smart and easy software. Simplified and optimized, key components, including an incubator and laser combiner, have been integrated into a self-contained package with no compromises. The system, which is easier to set up and use, features the same functionality as a high-end confocal laser scanning microscope while adding vibration isolation and a light tight cover. As a result, the FV10i can be operated by new and experienced users alike and, with a compactThe FV10i can be used with ease in a laboratory, unlike conventional confocal laser scanning microscopes which may require a dark room.Imaging can be performed in the conditions that are most suited for each fluorescence dye.The FV10i can capture images from 10.A vibration isolation table is not required and so can be installed directly on an experimental tabletop.Multi-stained specimens can be imaged with up to four fluorescence dyes. Maintenance-free and power-saving diode lasers with longer operating lives are employed in all the laser units, and operate with low noise levels.The fluorescent channels use a newly developed spectrum method comprising grating, beam splitter, and slit.

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In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The standard thickness of the cover glass is 0.17 mm, but the range from 0.13 to 0.21 mm is acceptable. Please prepare a WUXGA (1920 x 1200 pixels) display. Laser Scanning Microscopes. Confocal Laser Scanning Microscope Multiphoton Laser Scanning Microscope Super Resolution Microscopes. Super Resolution Microscope System Solutions-Based Systems. Research Slide Scanner Light Sheet Microscope. Resources Inverted Microscopes. Compound Microscope System Automated Microscope System Live Cell Imaging Microscope System. Overview TIRF Imaging Microscope System Confocal Imaging Microscope System Super Resolution Microscope System. Resources Routine Microscopes. IX53 Compact Cell Culture Microscopes Upright Microscopes. Fluorescence Microscopes. BX63 BX53 BX53 Clinical Microscopes. BX43 BX46 Fixed Stage Microscope Electrophysiology Microscopes. Fixed-Stage Post Microscope Fixed Stage Microscope Polarizing Microscope Asbestos Measurement Microscopes. Overview Specifications Resources Routine Microscopes. Research Stereo Microscope System. SZX16 SZX10 Stereo Microscope System Compact Stereo Microscopes Research Macro Zoom Microscope. Macro Zoom Fluorescence Microscope System Cell Culture Equipment. Incubation Monitoring System Automated Cell Counter Compact Inverted Microscopes Confluency Checker Cameras. Color Cameras ? DP74 DP27 DP22 UC90 SC180 SC50 EP50 LC30 XC10-IR CMOS Cameras Result of the operation test for DP camera-commercial PCs Monochrome Cameras. Mirror Units IMMOIL-F30CC Micrometers Light Sources. EasyLED VisiLED KL 1500 HAL KL 1600 LED KL 2500 LED KL 300 LED SZ2-CLS Lumencor Spectra X precisExcite U-HGLGPS U-LH100HG X-Cite 120 Iris X-Cite 120 PC X-Cite 120 Q X-Cite XLED1 Sakura Tissue Tek Histology Products OEM Components Applications. Life Science Research.

The user friendly navigation functions allow even a first-time user to capture images with ease.Three-dimensional images can be constructed.Appropriate imaging conditions can be set for each area.Specific regions can be selected for closer examination.Main settings include:The imaging area is determined through the framing and zooming functions. It is possible here to switch between the displays for each type of fluorescence dye.Just follow the navigational guidance to easily complete your imaging. Stitching Function Loading gallery. Loading. A map image of the entire glass slide can also be created.Also, the system is equipped with various display functions which allow a user to freely change the angle of 3D images and section the image at any spot.This software supports a wide range of well-used formats with high interchangeability including TIFF, BMP and JPEG.The FV10i motorized stage automatically moves to the oil supply position when switching to the oil immersion objective, allowing oil to be supplied efficiently without removing the specimen.Specimens can be observed without the risk of contamination using the closed contamination-free plastic cover of a dia. 35 mm glass bottom dish.These can be captured in high-definition and wide field of view.The images captured are stored automatically in the HDD. Large volumes of data, such as those obtained from long-term time-lapse imaging can be stored. An external HDD connected to a network can be specified as the destination, and the saved images can be viewed on a remote PC while performing separate imaging.If you do not change your web settings, cookies will continue to be used on this website. Laser Scanning Microscopes. Confocal Laser Scanning Microscope Multiphoton Laser Scanning Microscope Super Resolution Microscopes. Super Resolution Microscope System Solutions-Based Systems. Research Slide Scanner Light Sheet Microscope. Resources Inverted Microscopes.

In addition, they are equipped with the variable barrier filter function where the most suitable wavelength width is set automatically in accordance with the characteristics of the fluorescence dye.Images can be acquired through line sequences without crosstalk in imaging with two fluorescence dyes, and with three or four dyes in frame sequences with the virtual channel function.After that, the sophisticated user interface offers clear and efficient operation of the microscope. The selection of the imaging point, for example, can be performed easily using the newly designed image mapping menu without need for special experience or expertise. Furthermore, using advanced functionality that is only found in Olympus products, automatic focus and intensityIn addition, the system is equipped with a navigation function that identifies the operational step of the imaging procedure and guides the operator to the next appropriate operation. The FV10i confocal laser scanning microscope, therefore, provides a stress-free and comfortable operating environment even for first-time users.The FV10i automatically selects the most suitable imaging conditions based on the fluorescence dye selection.Users can then easily identify the desired image acquisition point.Image capture is then completed at the click of a button.The selectable map areas are automatically displayed in a diagram according to the type of specimen holder that has been used, such as a 35mm diameter dish or glass slide. An area can be selected and map image acquired by clicking on the desired scan area in the diagram. This will display an overview image of the area on the “Map Image” screen. Selecting an alternative area requires just aThese images can be displayed individually or be overlaid with each other.A Map Image of the Specimen Can be Automatically Created Loading gallery. Loading. High speed with low resolution or low speed with high resolution image mapping options is available.

The completely re-engineered design of this microscope, integrated into a self-contained package with a variety of functions, enables even inexperienced and first-time users to perform easily and efficient high-quality confocal imaging. Olympus has made no compromises in ergonomics and image quality, using high-quality optical components and smart and easy software. Simplified and optimized, key components, including an incubator and laser combiner, have been integrated into a self-contained package with no compromises. The system, which is easier to set up and use, features the same functionality as a high-end confocal laser scanning microscope while adding vibration isolation and a light tight cover. As a result, the FV10i can be operated by new and experienced users alike and, with a compactThe FV10i can be used with ease in a laboratory, unlike conventional confocal laser scanning microscopes which may require a dark room.Imaging can be performed in the conditions that are most suited for each fluorescence dye.The FV10i can capture images from 10.A vibration isolation table is not required and so can be installed directly on an experimental tabletop.Multi-stained specimens can be imaged with up to four fluorescence dyes. Maintenance-free and power-saving diode lasers with longer operating lives are employed in all the laser units, and operate with low noise levels.The fluorescent channels use a newly developed spectrum method comprising grating, beam splitter, and slit. In addition, they are equipped with the variable barrier filter function where the most suitable wavelength width is set automatically in accordance with the characteristics of the fluorescence dye.Images can be acquired through line sequences without crosstalk in imaging with two fluorescence dyes, and with three or four dyes in frame sequences with the virtual channel function.Time-lapse imaging up to a maximum of three days is supported.

Compound Microscope System Automated Microscope System Live Cell Imaging Microscope System. Overview TIRF Imaging Microscope System Confocal Imaging Microscope System Super Resolution Microscope System. Resources Routine Microscopes. IX53 Compact Cell Culture Microscopes Upright Microscopes. Fluorescence Microscopes. BX63 BX53 BX53 Clinical Microscopes. BX43 BX46 Fixed Stage Microscope Electrophysiology Microscopes. Fixed-Stage Post Microscope Fixed Stage Microscope Polarizing Microscope Asbestos Measurement Microscopes. Overview Specifications Resources Routine Microscopes. Research Stereo Microscope System. SZX16 SZX10 Stereo Microscope System Compact Stereo Microscopes Research Macro Zoom Microscope. Macro Zoom Fluorescence Microscope System Cell Culture Equipment. Incubation Monitoring System Automated Cell Counter Compact Inverted Microscopes Confluency Checker Cameras. Color Cameras ? DP74 DP27 DP22 UC90 SC180 SC50 EP50 LC30 XC10-IR CMOS Cameras Result of the operation test for DP camera-commercial PCs Monochrome Cameras. Mirror Units IMMOIL-F30CC Micrometers Light Sources. EasyLED VisiLED KL 1500 HAL KL 1600 LED KL 2500 LED KL 300 LED SZ2-CLS Lumencor Spectra X precisExcite U-HGLGPS U-LH100HG X-Cite 120 Iris X-Cite 120 PC X-Cite 120 Q X-Cite XLED1 Sakura Tissue Tek Histology Products OEM Components Applications. Life Science Research. Cancer Research Cell Culture Developmental Biology Drug Discovery Drug Discovery Fluorescence Live Cell Imaging Molecular Cell Biology Neuroscience Regenerative Medicine Clinical Research. Microscope Classroom Ask the Experts Learn about microscope (Japanese text only) Terminology Scientific Articles Video Articles Application Notes Microscopy Resource Center The Olympus Museum: Microscopes Support.

An external HDD connected to a network can be specified as the destination, and the saved images can be viewed on a remote PC while performing separate imaging.The upper panels in the slide shows that spheroids maintained their stem cell state as they were cultured without serum, leading to a halt in the cell cycle. On the other hand, in the lower panels, spheroids have been cultured with serum, which is responsible for cell division. The images indicate that these spheroids areThe confocal laser scanning microscope FV10i enabled the observation of thick spheroids in three dimensions.Clin Cancer Res. December 1, 2013 19:6495-6505.The clumps of cancer cells treated with cisplatin or radiation remained the same size and the majority of the cells did not proceed the cell cycle beyond the G1 phase (seen in red). On the other hand, the clump of cancer cells treated with telomelysin showed a change in color from red toThis indicates that telomelysin inhibits the expression of p53 and p21, both of which are responsible for arresting the cell cycle of stem-like cells. Telomelysin also increases the expression of E2F-1, which leads to the activation of the cell cycle by contraries.Clin Cancer Res. December 1, 2013 19:6495-6505.Nature 496, 363-366 (18 April 2013)J Cell Biol. 197(4):553-568(2012).Image is comprised of 9 different regions of interest automatically acquired over 14 Z sections at 1024x1024 and stitched together using FV10i software.If you do not change your web settings, cookies will continue to be used on this website. My Bench Close Not a Member.You can adjust your Community Subscriptions in Settings Continue Removed. FV10i is the world's first self-contained Confocal Laser Scanning Microscope. We have completely re-engineered the design of the confocal laser scanning microscope into a self-contained package integrated with a variety of functions including an incubator and a laser combiner.

You can install the FV10i easily in a laboratory without having to prepare a dedicated room. The FV10i also has unique features like a vibration isolation function, and light-tight cover, eliminating the need for a dark room. The FV10i has the same standard functionality of a high end confocal laser scanning microscope, with easy-to-use software delivering it in a compact design. The system is equipped with four lasers, which means multi-stained specimens can be imaged with up to four fluorescence dyes. Maintenance-free and power-saving diode lasers with longer operating lives are employed in all the laser units, and operate with low noise levels. The detecting mechanism has two fluorescence channels, and one phase contrast channel. The fluorescent channels use a newly developed spectrum method comprising grating, beam splitter, and slit. In addition, they are equipped with the variable bandpass filter function where the most suitable wavelength width is set automatically in accordance with the characteristics of the fluorescence dye. You can acquire images through line sequences without crosstalk in imaging with two fluorescence dyes, and with three or four dyes in frame sequences with the virtual channel function. Zoom magnification can be changed continually from 10x to 600x. The most suitable imaging area can be set depending on size of the specimen. The system is equipped with specimen holders, usable for a 35mm glass bottom dishes, glass slides, and cover glass chambers (8-well type). You can observe the specimen worry-free with the closed contamination-free plastic cover of a 35mm glass bottom dish. Imaging of adjacent images 2x2 and 3x3 is possible. You can capture images of high-definition and wide-field of view. The microscope comes equipped with a HDD (hard-disk drive) recording function. The images captured are stored automatically. Large volumes of data, such as those obtained from long-term time-lapse imaging can be saved.

Recirculation of the culture media and addition of a medicinal solution during time-lapse is possible. In addition, the culture pod system can be autoclaved for sterilization.Long term time-lapse imaging can continue without concern for immersion media levels. Water is supplied automatically when the objective is moved into the observation position.This assures imaging is performed each time with optimal conditions.Ten point locations can be assigned within a single dish (well). For example, in the case of a dia. 35mm glass bottom dish, three dishes can be mounted, allowing a maximum of up to 30 locations to be captured.After that, the sophisticated user interface offers clear and efficient operation of the microscope. The selection of the imaging point, for example, can be performed easily using the newly designed image mapping menu without need for special experience or expertise. Furthermore, using advanced functionality that is only found in Olympus products, automatic focus and intensityIn addition, the system is equipped with a navigation function that identifies the operational step of the imaging procedure and guides the operator to the next appropriate operation. The FV10i confocal laser scanning microscope, therefore, provides a stress-free and comfortable operating environment even for first-time users.The FV10i automatically selects the most suitable imaging conditions based on the fluorescence dye selection.Users can then easily identify the desired image acquisition point.Image capture is then completed at the click of a button.With this bird's-eye view of the specimen, the user can quickly and easily select the imaging area to be captured. Create Map Area The selectable map areas are automatically displayed in a diagram according to the type of specimen holder that has been used, such as a 35 mm diameter dish or glass slide. An area can be selected and map image acquired by clicking on the desired scan area in the diagram.

This will display an overview image of the area on the “Map Image” screen. Selecting an alternative area requires just aThese images can be displayed individually or be overlaid with each other. Scanning Setting High speed with low resolution or low speed with high resolution image mapping options is available.The user friendly navigation functions allow even a first-time user to capture images with ease. Observation Mode Selection Z-stack In Z-stack mode, images are repeatedly acquired in different focus positions. Three-dimensional images can be constructed. Z-stack time-lapse Imaging which integrates Z-stack and time-lapse are acquired. Multi-area time-lapse Time-lapse imaging is performed automatically at pre-selected points. Multi-area Z-stack time-lapse Imaging where all three functions are performed. Multi-area Setting Appropriate imaging conditions can be set for each area.Specific regions can be selected for closer examination.Main settings include:The imaging area is determined through the framing and zooming functions. It is possible here to switch between the displays for each type of fluorescence dye.Just follow the navigational guidance to easily complete your imaging.A map image of the entire glass slide can also be created.Also, the system is equipped with various display functions which allow a user to freely change the angle of 3D images and section the image at any spot.This software supports a wide range of well-used formats with high interchangeability including TIFF, BMP and JPEG.Specimens can be observed without the risk of contamination using the closed contamination-free plastic cover of a dia. 35 mm glass bottom dish.These can be captured in high-definition and wide field of view.The images captured are stored automatically in the HDD. Large volumes of data, such as those obtained from long-term time-lapse imaging can be stored.

During imaging, editing and analysis of previously taken images is possible. You can also specify an external HDD connected to a network, and you can view the saved images on a remote PC while performing separate imaging. General Specifications Microscope Type Confocal Looking for New or Used Equipment. Shop on LabX Shop on Labx.com This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies. Please review our Privacy Policy for more details. All Rights Reserved. They make advanced imaging accessible to all, without compromising on quality or speed. Empowering technology Making advanced microscopy more inclusive requires an exact combination of. Imagine if this could be done for complex scientific techniques such as microscopy. By seamlessly coupling high-quality microscopy and imaging hardware with precision automation and user-centric software within the FSX100 and FluoView FV10i all-in-one microscope imaging systems, Olympus has achieved this exceptional feat. This image shows a close-up of a bird’s wing Multichannel fluorescence microscopy, for instance, has provided insights into the localisation, colocalisation and function of many different proteins and other molecules across a large number of cell types. Confocal microscopy has taken this to another level, providing increased clarity and resolution. Controlling microscope systems capable of such imaging, though, often takes much practice and. The speed and ease with which fluorescence and confocal laser scanning microscopy images can be created not only makes the FSX100 and FV10i ideal for beginners, but also offers experienced users unique capabilities. For example, the FV10i water immersion model is fitted with an incubator, ensuring that long-term live cell confocal microscopy can be carried out. Furthermore, a broader range of scientists are turning to fluorescence microscopy to see what is happening at the cellular and subcellular levels.

As a result, not everyone that uses a microscope has the experience to generate good images on their own and therefore skilled users are called upon for assistance, impacting on their time. The FSX100 all-in-one fluorescence microscope from Olympus ensures. As a result, there is no requirement for a dedicated phase-contrast objective and the phase ring is only placed in the lightpath when needed. Specimen holder C D The FSX100 supports a number of different specimen formats including stand-. The Olympus FV10i is a confocal laser scanning microscope and is available in either oil or water immersion models. Its compact design is possible due to Olympus’ excellence in optoelectronic design and implementation. Unique confocal system B The FV10i features 4 laser diodes (405, 473, 559 and 635 nm), carefully arranged in a compact laser combiner housed within the body of the FV10i. The. To further ensure that the FV10i instruments can be placed directly at the point of discovery, once a sample is loaded and the lid shut, the imaging compartment doubles up as a darkroom, increasing the efficiency of confocal imaging so that laser intensities can be minimised and even the weakest of signals can be detected. Motorised stage Integrated and incubated darkroom on a. Astonishingly, each bird knows intuitively where they are heading and when to turn or swoop. The Olympus all-in-one box microscope range enables all users, even those who have never used a microscope before, to have a similar intuitive, almost a priori, knowledge of how to achieve the best images from their samples. The “set, select, capture” concept enables the user to load their sample and then be guided through a straightforward acquisition. This not only ensures that a broader range of people can access more advanced imaging techniques, but that they can bring an extra dimension to their research, expanding the tools they use, and as a result discover more. Once loaded, the user selects the imaging mode.

Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates. You may have to register before you can post: click the register link above to proceed. To start viewing messages, select the forum that you want to visit from the selection below. The completely re-engineered design of this microscope, integrated into a self-contained package with a variety of functions, enables even inexperienced and first-time users to perform easily and efficient high-quality confocal imaging. Olympus has made no compromises in ergonomics and image quality, using high-quality optical components and smart and easy software. The FV10i features a unique self-contained design. Simplified and optimized, key components, including an incubator and laser combiner, have been integrated into a self-contained package with no compromises. The system, which is easier to set up and use, features the same functionality as a high-end confocal laser scanning microscope while adding vibration isolation and a light tight cover. As a result, the FV10i can be operated by new and experienced users alike and, with a compact footprint, can be installed in any laboratory without the need for a dedicated dark room. High-definition Images using Advanced Optics Built-in Incubator Ideal for Time-lapse Imaging Stress-free Operation for Every User Only two manual steps are required of the user: placing of the specimen on the stage and closing the cover. After that, the sophisticated user interface offers clear and efficient operation of the microscope. Capture a Map Image Easily for Navigation When the Specimen Has Been Loaded, Clicking Begins the Mapping Process Easy Guided Operation for First-time Users Sophisticated Menus Allow Easy Navigation of Imaging Area Dedicated Analysis Software Exclusive to FLUOVIEW Feature-rich Functionality for Stress-free Imaging Model: FV10C-O Power: 240V Serial: 9D08770.

With the FV10i, I got all that and more—superior I needed a system that would give me the same high-end imaging as a confocal, but would fit on my benchtop. With the FV10i, I got all that and more—superior image quality, a compact solution, and an easy-to-use instrument—with no darkroom. Write Review All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information: If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance. Use the form below to email us your questions about products, online orders, store experiences and more. Give us a call, or drop us a line and let us know what you're looking for as best you can. We're always adding partner brands, and it's difficult to keep up with the thousands of products that get added to our lineup. Our sales specialists know our lineup best, and are most willing to help you find the solution(s) you need! If you find that you can't complete an order, contact us via phone or email for further assistance. Not only do we accept most major credit cards (Visa, Master Card, Discover, American Express), we also offer you the flexibility of paying by check, money order, wire transfers, and purchase orders. Work with any one of our sales specialists to figure out which method is best for you!

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